Modulation by biogenic amines for the hemocyte count and prophenoloxidase exocytosis via receptors in Litopenaeus vannamei

Hemocyte counts and phenoloxidase(PO)activity were examined after hemolymph being incubated in dopamine(DA),noradrenaline(NE)and serotonin(5-HT).Results showed that all the three biogenic amines(BAs)had a significant impact on total hemocyte count(THC),differential hemocyte count(DHC),and intracelluar and extracelluar phenoloxidase(PO)activity.Among these Bas,DA had the strongest effect on the above parameters,whereas 5-HT had the least effect.Preincubation with D1 receptor antagonist SCH23390,D2 receptor antagonist Sulpiride and 1:1 admixture of the two could significantly inhibit the effect of DA on these parameters.SCH23390 showed a stronger inhibitory effect than Sulpiride,and the admixture exhibited the strongest effect.These results suggested that the change of hemocyte count and activation of prophenoloxidase(proPO)system in Litopenaeusvan-namei hemocyte can be regulated by BAs,and DA modulates the two parameters via its receptors.     

Structure analysis of the complete mitochondrial genome in cultivation variety ��Rongfu��

In this report, complete mitochondrial genome sequences of Laminaria cultivation variety ‘Rongfu’ were obtained. The results showed the length of circular molecule of mtDNA was 37 638 bp (64.7% A+T), encoding three rRNAs (23S, 16S and 5S), 25 tRNAs, 35 known mitochondrial proteins and 3 ORFs. Sequence alignment indicated its mtDNA genome was very similar to that of Laminaria japonica. Phylogenetic trees inferred from concatenated 30 mitochondrial genes showed that ‘Rongfu’, Laminaria japonica, Laminaria longipedalis, Laminaria diabolica, Laminaria religiosa and Laminaria ochotensis clustered together. In addition, compared with mitochondrial genome of L. japonica, ‘Rongfu’ mtDNA lacked a non-coding region of 19 nucleotides, which was located between rRNA small subunit gene 3 (rps3) and rRNA small subunit gene 9 (rps9). Seven cultivation varieties of China were divided into two groups based on this non-coding region which was absent in ‘Rongfu’, ‘Fujian’ and ‘Sanhai’ while present in ‘Ailunwan’, ‘Dongfang No.2’, ‘Dongfang No.3’ and ‘Zaohoucheng’. So this variation can be used in germplasm identification of cultivation variety.     

Numerical study on the bifurcation of the North Equatorial Current

A 1.5-layer reduced-gravity model forced by wind stress is used to study the bifurcations of the North Equatorial Current(NEC).The authors found that after removing the Ekman drift,the modelled circulations can serve well as a proxy of the SODA circulations on the σθ=25.0 kg m～-3 potential density surface based on available long-term reanalysis wind stress data.The modelled results show that the location of the western boundary bifurcation of the NEC depends on both zonal averaged and local zero wind stress curl latitude.The effects of the anomalous wind stress curl added in different areas are also investigated and it is found that they can change the strength of the Mindanao Eddy(ME),and then influence the interior pathway.     

A study of extratropical transition and re-intensification of Typhoon Mindulle (2004)

In this study,the fifth-generation Pennsylvania State University-National Center for Atmospheric Research (PSU-NCAR) Mesoscale Model (MM5) is used to simulate Typhoon Mindulle (2004) at high resolution (3-km grid size).The data from measurements show that in the upper atmosphere the existence of an upper jet is important to the transition cyclone.When Mindulle moved to the area of the upper jet entrance,where high-altitude divergence existed, the pumping of the high-altitude divergence would enhance the vertical motion and low-level cyclone convergence. The enhanced vertical motion was confirmed by the simulation results and indicated that the existence of upper divergence enhanced the vertical motion which was favorable for the maintenance of Typhoon Mindulle.The process of extratropical transition (ET) and re-intensification always accompanies the process of cold air invasion. This process enhances the baroclinicity of the atmosphere and the formation of front at high altitudes, which converts baroclinic potential energy into kinetic energy and strengthens the cyclone vortex.The distributions of equivalent potential temperature (θe) and temperature anomalies show that the warm-core of the typhoon at the tropopause aids the re-intensification of the system. As the typhoon reenters the ocean, latent heat flux (LHF) increases in the north and west and the strong reflectivity and vertical motion occur in the east and southeast,and the west.With the re-intensification of the typhoon the wind field evolves from an oval to a circle at the lower atmosphere, the area coverage by high winds increases, and the distribution of the tangential wind shows an asymmetric pattern.     

Effects of heavy metal ions (Cu2+, Pb2+ and Cd2+) on DNA damage of the gills, hemocytes and hepatopancreas of marine crab, Charybdis japonica

There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment.     

郑州节水型社会试点建设评价研究

郑州市是水利部确定的南水北调东中线受水区全国节水型社会建设试点,自2005年试点建设开展以来取得了显著的成绩,被授予全国节水型社会建设示范市。为科学评价试点建设成效,参照水利部制定的《节水型社会建设评价指标体系(试行)》,结合郑州市实际,建立了郑州节水型社会试点建设评价指标体系。采用层次分析法对节水型社会试点建设成效进行了评价,确定郑州市已基本建立节水型社会。     

统筹城乡发展导向的中心村镇建设理论思考

统筹城乡发展战略为促进乡村空间重构、优化城乡格局提出了新要求。中心村镇是连接城市和散乱村庄建设的纽带,通过推进一定乡村地域的组织、产业和空间"三整合",可以有效利用各种资源,促进农村经济、社会和环境的协调与健康发展,中心村镇建设的科学规划与决策也亟需相关理论支撑。以乡村地理学、乡村规划学和乡村社会学等领域为背景,着眼于空间、时间和主体3个维度,对增长极理论、中心地理论、农业区位论、经济成长阶段论、空心村生命周期理论、社区(公众)参与等中心村镇建设相关理论进行梳理分析,探讨了这些理论在我国中心村镇建设与规划中的应用,认为中心村镇建设需要加强基础理论研究,分类指导我国中心村镇建设的实践。     

中部六省R&D经费投入与结构分析

以中部六省为研究对象,对R&D经费投入总量及强度、R&D经费的支出结构与来源结构进行深入分析,并找出与先进地区或国家相比存在的差距。研究结果表明:中部六省之间,R&D经费投入与结构均存在较大差异。总体而言,中部六省R&D经费投入不足,强度偏低;对原始创新重视程度不够,R&D经费支出在科研机构与高校所占的比例以及在基础研究与应用研究所占的比例偏低;政府对R&D活动支持的力度有待进一步提升。     

城市景观公园对城市热岛调控效应分析——以哈尔滨市为例

采用哈尔滨市2007年9月30日Landsat TM数据,基于哈尔滨市地表温度反演结果,对城市公园对热岛效应的影响进行了分析。研究结果表明:哈尔滨市主城区热岛效应明显,高温区呈现集中分布,市区内的公园在高温区域内形成明显的热岛空洞;公园对周围区域降温作用明显,降温效应随距离增加呈递减趋势;在公园周围120 m范围内,不同面积的公园对周围环境温度影响存在明显差异,不同缓冲带的降温幅度存在极显著差异;公园面积和内部平均温度呈显著负相关,即公园面积越大,内部平均温度越低,反之亦然,二者存在显著的回归关系,因此可以通过设计不同面积公园,以实现城市高温区降温效应。研究表明一个大的景观公园的降温效应要强于总面积相等的多个小公园的降温效应。     

大城市边缘区农村居民点用地空间布局优化研究——对上海南汇地区的考察

大城市边缘区农村居民点用地存在用地粗放、布局散乱、难于规范管理等问题。以大城市边缘区农村居民点用地整理为契机,优化空间布局不仅有利于形成农村经济新增长点,还可促进农村居民点土地集约节约利用。在分析当地农村居民点用地现状及其分布状况的基础上,以典型地区——上海南汇地区为例,对其空间分布的随机性进行了检验,对农村居民点用地按标准偏差分类,并进一步按欧氏空间距离对大于2.25标准偏差的地块进行聚类分析。结果显示:南汇地区农村居民点用地的空间分布并不是随机分布,可分为远离中心类和集聚中心类两种类型。依据结果,提出了"远离中心方案"和"集聚中心方案"两种不同的农村居民点用地整理方案。